Integrative Vectors for Gene Deletion and Replacement
نویسندگان
چکیده
منابع مشابه
Integrative vectors for gene deletion and replacement.
An improved method for gene deletion or replacement in Escherichia coli was developed. It employs a set of integrative vectors and two helper plasmids, as a temporary source of RecA and Flp activities. The integrative vectors combine several useful features including three different selection markers placed between two parallel oriented Flp recombinase target (FRT) sites. Each marker is flanked...
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1.Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580. 2.Ishii, T.M., P. Zerr, X.M. Xia, C.T. Bond, J. Maylie and J.P. Adelman. 1998. Site-directed mutagenesis. Methods Enzymol. 293:53-71. 3.Kirsch, R.D. and E. Joly. 1998. An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes. Nucleic Acids R...
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ژورنال
عنوان ژورنال: Polish Journal of Microbiology
سال: 2013
ISSN: 2544-4646,1733-1331
DOI: 10.33073/pjm-2013-010